|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15439||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1 N-FLAG/Hygro
Backbone manufacturerConaway Lab
- Backbone size (bp) 5600
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Protein
- FLAG (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer BGH rev (Common Sequencing Primers)
Terms and Licenses
pcDNA3.1/Hygro (from Invitrogen) was modified to include an N-terminal FLAG tag. See Author's Map for cloning details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1 N-FLAG/Hyg(+) was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 15439)
Map uploaded by the depositor.