Addgene - -948 hTF Plasmid Data

Plasmid 15441: -948 hTF

Gene/insert name:
Tissue factor promoter
Alt name:
TF
Insert size:
1069
Species:
H. sapiens (human)
Entrez Gene:
F3 (CD142, TF, TFA)
Vector backbone:
pGL2 Basic
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
5600
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
KpnI
Site destroyed during cloning:
No
5' sequencing primer:
na List of Sequencing Primers
3' sequencing primer:
LucNrev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Nigel Mackman
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

-948 to +121 hTF promoter. hTF promoter deletion series, Mackman lab #2493.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Regulation of the tissue factor gene. Mackman et al (FASEB J. 1995 Jul . 9(10):883-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15441" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

15443: -227 hTF (mutant AP-1)