-111 hTF m4
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15451||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepGL2 Basic
- Backbone size w/o insert (bp) 5600
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameTissue factor promoter
SpeciesH. sapiens (human)
Insert Size (bp)125
MutationAll 3 Sp1 and EGR-1 sites mutated
Entrez GeneF3 (a.k.a. CD142, TF, TFA)
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer na
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
-111 to +14 hTF promoter with all 3 Sp1 and EGR-1 sites mutated. hTF promoter deletion/mutation series 2, Mackman lab #2436.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:-111 hTF m4 was a gift from Nigel Mackman (Addgene plasmid # 15451 ; http://n2t.net/addgene:15451 ; RRID:Addgene_15451)
For your References section:Transcriptional regulation of the tissue factor gene in human epithelial cells is mediated by Sp1 and EGR-1. Cui MZ, Parry GC, Oeth P, Larson H, Smith M, Huang RP, Adamson ED, Mackman N. J Biol Chem. 1996 Feb 2. 271(5):2731-9. 10.1074/jbc.271.5.2731 PubMed 8576248