PurposeMoClo Level 1 luciferase reporter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||154706||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeSynthetic Biology
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameMinSyn_013 Firefly luciferase expression cassette [MinSyn_013::TMV::LucF::Flag::ocsT]
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
Please visit https://www.biorxiv.org/content/10.1101/2020.05.14.095406v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEPYC1CB0450 was a gift from Nicola Patron (Addgene plasmid # 154706 ; http://n2t.net/addgene:154706 ; RRID:Addgene_154706)
For your References section:Rational design of minimal synthetic promoters for plants. Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ. Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. 10.1093/nar/gkaa682 PubMed 32856047