pBBR_Cas9_sp1
(Plasmid #154851)

• Purpose: Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome
• Depositing Lab: John van der Oost
• Publication: Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204. doi: 10.1186/s12934-019-1255-1.

Backbone

• Vector backbone: pBBRMCS2
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: The plasmid should be transformed to E. coli S17-1, the vector donor strain for R. sphaeroides in diparental conjugation.
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Spcas9 gene
• Species: Streptococcus pyogenes
• Insert Size (bp): 4122
• Mutation: Codon harmonized variant for expression in Rhodobacter sphaeroides
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: sgRNA scaffold
• Species: Streptococcus pyogenes
• Insert Size (bp): 79

Resource Information

• Supplemental Documents:
  • pBBR_Cas9_sp1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid should be transformed to E. coli S17-1, the vector donor strain for R. sphaeroides in diparental conjugation.