|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15537||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneE61 (pBS derivative)
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1000
Entrez GeneDlx2 (a.k.a. AW121999, Dlx-2, Tes-1)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
To make antisense probe, cut with HindIII for full-length probe or EcoRI for 560 bp probe without the homeobox, and transcribe with T3 RNA polymerase. To make sense probe, cut with NotI and transcribe with T7 RNA polymerase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Dlx2 was a gift from John Rubenstein (Addgene plasmid # 15537 ; http://n2t.net/addgene:15537 ; RRID:Addgene_15537)
For your References section:Isolation and characterization of a novel cDNA clone encoding a homeodomain that is developmentally regulated in the ventral forebrain. Porteus MH, Bulfone A, Ciaranello RD, Rubenstein JL. Neuron. 1991 Aug . 7(2):221-9. 10.1016/0896-6273(91)90260-7 PubMed 1678612