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pKH3-p190
(Plasmid #15547)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15547 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p190 GTPase activating protein
  • Alt name
    p190
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    4500
  • GenBank ID
    M94721
  • Entrez Gene
    Arhgap35 (a.k.a. Grlf1, p190RhoGAP)
  • Tag / Fusion Protein
    • Triple HA1 tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The HindIII-EcoRI fragment used to produce the plasmid was isolated from p190 cDNA clone 391 provided by R. Weinberg and J. Settleman (Massachusetts Institute of Technology).
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Digesting the plasmid with BamHI+EcoRI will excise the entire full-length p190 ORF.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKH3-p190 was a gift from Ian Macara (Addgene plasmid # 15547 ; http://n2t.net/addgene:15547 ; RRID:Addgene_15547)
  • For your References section:

    The function of the p190 Rho GTPase-activating protein is controlled by its N-terminal GTP binding domain. Tatsis N, Lannigan DA, Macara IG. J Biol Chem. 1998 Dec 18. 273(51):34631-8. 10.1074/jbc.273.51.34631 PubMed 9852136