|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15714||Plasmid sent as bacteria in agar stab||1||$65||Add to Cart|
- Backbone size w/o insert (bp) 7200
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Alt nameC/EBP beta isoform
SpeciesH. sapiens (human)
Insert Size (bp)500
Entrez GeneCEBPB (a.k.a. C/EBP-beta, IL6DBP, NF-IL6, TCF5)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer LNCX (Common Sequencing Primers)
Human LIP was cloned by PCR from cDNA templates and then subsequently digested with BamHI, inserted in pQCXIP (Clontech), and analyzed for the right orientation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pQCXIP-LIP was a gift from Joan Massague (Addgene plasmid # 15714)
For your References section:C/EBPbeta at the core of the TGFbeta cytostatic response and its evasion in metastatic breast cancer cells. Gomis RR, Alarcon C, Nadal C, Van Poznak C, Massague J. Cancer Cell. 2006 Sep . 10(3):203-14. 10.1016/j.ccr.2006.07.019 PubMed 16959612
Map uploaded by the depositor.