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pQCXIP-LIP
(Plasmid #15714)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pQCXIP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    LIP
  • Alt name
    C/EBP beta isoform
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    500
  • Entrez Gene
    CEBPB (a.k.a. PP9092, C/EBP-beta, IL6DBP, NF-IL6, TCF5)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer LNCX
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Human LIP was cloned by PCR from cDNA templates and then subsequently digested with BamHI, inserted in pQCXIP (Clontech), and analyzed for the right orientation.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXIP-LIP was a gift from Joan Massague (Addgene plasmid # 15714)
  • For your References section:

    C/EBPbeta at the core of the TGFbeta cytostatic response and its evasion in metastatic breast cancer cells. Gomis RR, Alarcon C, Nadal C, Van Poznak C, Massague J. Cancer Cell. 2006 Sep . 10(3):203-14. 10.1016/j.ccr.2006.07.019 PubMed 16959612