Addgene - pET15-UbE2D1 Plasmid Data

Plasmid 15782: pET15-UbE2D1

Gene/insert name:
Ubiquitin conjugating enzyme UbE2D1
Alt name:
UbE2D1
Insert size:
444
Species:
H. sapiens (human)
GenBank ID:
NM_003338
Entrez Gene:
UBE2D1 (E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A)
Fusion protein or tag:
His
Terminal:
N terminal on backbone
Vector backbone:
pET15
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5700
Cloning site 5':
NdeI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Wade Harper
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Jin et al (Nature. 2007 Jun 28. 447(7148):1135-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15782" in your Materials and Methods section.