Addgene - pDEST17-UbE2S Plasmid Data

Plasmid 15807: pDEST17-UbE2S

Gene/insert name:
Ubiquitin conjugating enzyme UbE2S
Alt name:
UbE2S
Insert size:
669
Species:
H. sapiens (human)
GenBank ID:
NM_014501
Entrez Gene:
UBE2S (EPF5, E2EPF, E2-EPF)
Fusion protein or tag:
His
Terminal:
N terminal on backbone
Vector backbone:
pDEST17
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
4900
Cloning method:
Gateway Cloning
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (2)
Principal Investigator:
Wade Harper
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Jin et al (Nature. 2007 Jun 28. 447(7148):1135-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15807" in your Materials and Methods section.