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pDEST17-UbE2S
(Plasmid #15807)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pDEST17
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Ubiquitin conjugating enzyme UbE2S
  • Alt name
    UbE2S
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    669
  • GenBank ID
    NM_014501
  • Entrez Gene
    UBE2S (a.k.a. OK/SW-cl.73, E2-EPF, E2EPF, EPF5)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST17-UbE2S was a gift from Wade Harper (Addgene plasmid # 15807)
  • For your References section:

    Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Jin J, Li X, Gygi SP, Harper JW. Nature. 2007 Jun 28. 447(7148):1135-8. 10.1038/nature05902 PubMed 17597759