pMH-06
(Plasmid
#158434)
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PurposepBAD33 vector encoding MotA, Dendra2-MotB
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158434 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBAD33
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature30°C
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Growth Strain(s)JPA605 (parent strain RP437)
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Growth instructionsPlasmid was deposited to Addgene in a custom bacterial strain, JPA605 (parent strain RP437). However, the plasmid may easily be stored and expressed in DH5alpha.
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameMotA
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SpeciesE. coli
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Insert Size (bp)888
Gene/Insert 2
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Gene/Insert nameMotB
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SpeciesE. coli
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Insert Size (bp)927
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Tag
/ Fusion Protein
- Dendra2 (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMH-06 was a gift from Francesco Pedaci (Addgene plasmid # 158434 ; http://n2t.net/addgene:158434 ; RRID:Addgene_158434) -
For your References section:
Impact of fluorescent protein fusions on the bacterial flagellar motor. Heo M, Nord AL, Chamousset D, van Rijn E, Beaumont HJE, Pedaci F. Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.1038/s41598-017-11241-w. 10.1038/s41598-017-11241-w PubMed 28974721
