pMXs-Atoh1
(Plasmid
#158529)
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PurposeExpresses mouse Atoh1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158529 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMXs-GW
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsSince viral vectors are prone to recombination, we recommend testing 2-4 colonies to help select the full plasmid.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAtonal BHLH Transcription Factor 1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1056
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Entrez GeneAtoh1 (a.k.a. Hath1, MATH-1, Math1, bHLHa14)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pMX-S1811 (5'-GACGGCATCGCAGCTTGGATACAC-3') (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMXs-Atoh1 was a gift from Neil Segil (Addgene plasmid # 158529 ; http://n2t.net/addgene:158529 ; RRID:Addgene_158529) -
For your References section:
Generation of inner ear hair cells by direct lineage conversion of primary somatic cells. Menendez L, Trecek T, Gopalakrishnan S, Tao L, Markowitz AL, Yu HV, Wang X, Llamas J, Huang C, Lee J, Kalluri R, Ichida J, Segil N. Elife. 2020 Jun 30;9. pii: 55249. doi: 10.7554/eLife.55249. 10.7554/eLife.55249 PubMed 32602462
