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pGolf-ISH
(Plasmid #15853)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15853 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM-T Easy
  • Backbone manufacturer
    promega
  • Backbone size w/o insert (bp) 3015
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Guanine Nucleotide Binding Protein, Alpha Stimulating, Olfactory type
  • Alt name
    Golf
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1130
  • Mutation
    F199S
  • GenBank ID
    NM_010307
  • Entrez Gene
    Gnal (a.k.a. 2610011C15Rik, 9630020G10Rik, Galphaolf, Gna10, Golf)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

This plasmid was used to prepare RNA probes for in situ hybridization (ISH) and has not been used for functional studies of Gnal. It is not known whether the F199S mutation may affect function of Gnal. Moreover, be advised that the plasmid does not contain the full coding region of Gnal.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGolf-ISH was a gift from Peter Mombaerts (Addgene plasmid # 15853 ; http://n2t.net/addgene:15853 ; RRID:Addgene_15853)
  • For your References section:

    Differential impact of Lhx2 deficiency on expression of class I and class II odorant receptor genes in mouse. Hirota J, Omura M, Mombaerts P. Mol Cell Neurosci. 2007 Apr . 34(4):679-88. 10.1016/j.mcn.2007.01.014 PubMed 17350283