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V2rf2-IRES-BL Targeting Vector
(Plasmid #15860)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15860 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBS-SK+ with modification of PmeI and AscI into KpnI
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    V2rf2
  • Alt name
    mV2R58
  • Alt name
    EC1-V2R
  • Alt name
    Vmn2r81
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    13505
  • Entrez Gene
    Vmn2r81 (a.k.a. V2rf2, EC1-V2R, EC1-VR2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    V2rf2-IRES-BL Targeting Vector was a gift from Peter Mombaerts (Addgene plasmid # 15860 ; http://n2t.net/addgene:15860 ; RRID:Addgene_15860)
  • For your References section:

    Expression of nonclassical class I major histocompatibility genes defines a tripartite organization of the mouse vomeronasal system. Ishii T, Mombaerts P. J Neurosci. 2008 Mar 5. 28(10):2332-41. 10.1523/JNEUROSCI.4807-07.2008 PubMed 18322080