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Jeff Mumm
Sharrock et al
5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2
(Plasmid #158653)

Purpose

making transgenics expressing a UAS driven mCherry and NTR 2.0
Depositing Lab

Jeff Mumm
Publication

Sharrock et al bioRxiv ( How to cite )
Sequence Information
- Sequences (2)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	158653	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
(na) pTol2mini?
(Search Vector Database)
Vector type
Tol2-based transgenic expression in zebrafish/other

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Gene/Insert

Gene/Insert name
5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
Species
Escherichia coli
Mutation
F70A/F108Y

Resource Information

Terms and Licenses
- UBMTA
- Takara Bio Limited Use Label License (formerly Clontech)
Industry Terms
- Not Available to Industry

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2 was a gift from Jeff Mumm (Addgene plasmid # 158653 ; http://n2t.net/addgene:158653 ; RRID:Addgene_158653)
For your References section:

NTR 2.0: a rationally-engineered prodrug converting enzyme with substantially enhanced efficacy for targeted cell ablation. Sharrock AV, Mulligan TS, Hall KR, Williams EM, White DT, Zhang L, Matthews F, Nimmagadda S, Washington S, Le K , Meir-Levi D, Cox OL, Saxena MT, Calof AL, Lopez-Burks ME, Lander AD, Ding D, Ji H, Ackerley DF, Mumm JS. bioRxiv 10.1101/2020.05.22.111427

5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2 (Plasmid #158653)