PurposeVector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||158988||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepX601 (Addgene #61591)
- Total vector size (bp) 7033
Modifications to backbonereplaced CMV promoter with pMecp2; replace bGHpA with spA; replaced SaCas9 with dSaCas9-KRAB
Vector typeMammalian Expression, AAV, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Insert Size (bp)3494
- Promoter pMecp2
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer GCAGGTTGTAGTCGAACAGCAGCT
- 3′ sequencing primer CAGCACAGACATTCTGGGCAACCT (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA was a gift from Chung Tin (Addgene plasmid # 158988 ; http://n2t.net/addgene:158988 ; RRID:Addgene_158988)
For your References section:Targeted Transgene Activation in the Brain Tissue by Systemic Delivery of Engineered AAV1 Expressing CRISPRa. Lau CH, Ho JW, Lo PK, Tin C. Mol Ther Nucleic Acids. 2019 Jun 7;16:637-649. doi: 10.1016/j.omtn.2019.04.015. Epub 2019 Apr 23. 10.1016/j.omtn.2019.04.015 PubMed 31108320