Addgene - LTRi Plasmid Data

Plasmid 15913: LTRi

Gene/insert name:
None
Vector backbone:
LTRi
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size (bp):
8062
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
Stratagene SURE Very low copy--TLD recommends megapreps
High or low copy:
Low Copy
Sequence: View sequences (4)
Map:
View map
Supplemental document:
LTRi protocol (application/pdf)
Principal Investigator:
James Collins
Terms and Licenses:
MTA

Comments:

We engineered a tunable genetic switch called LTRi, which stands for Lac-Tet-RNAi. Gene expression is turned on by adding an inducer, isopropyl-β-thiogalactopyranoside (IPTG). See article for details.

Clone your gene of interest into the Transgene Module http://www.addgene.org/15916 and then move it into LTRi.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells. Deans et al (Cell. 2007 Jul 27. 130(2):363-72. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 15913" in your Materials and Methods section.