pQE30-VRN1
(Plasmid #159531)

• Purpose: Expresses Arabidopsis thaliana VERNALIZATION1 in E. coli
• Depositing Lab: Joshua Mylne
• Publication: King et al J Biol Chem. 2013 Feb 1;288(5):3198-207. doi: 10.1074/jbc.M112.438572. Epub 2012 Dec 19.

Backbone

• Vector backbone: pQE30
• Backbone manufacturer: Qiagen
• Backbone size w/o insert (bp): 3433
• Total vector size (bp): 3845
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: VERNALIZATION1
• Alt name: VRN1
• Alt name: At3g18990
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 411
• Entrez Gene: VRN1 (a.k.a. AT3G18990, REDUCED VERNALIZATION RESPONSE 1, REM39, REPRODUCTIVE MERISTEM 39)
• Promoter: T5
• Tag / Fusion Protein:
  • Six-Histidine tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CCCGAAAAGTGCCACCTG
• 3′ sequencing primer: GTTCTGAGGTCATTACTGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry