pCSJ392
(Plasmid
#159722)
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PurposeExpress Gid10 (YGR066C) in yeast two hybrid plasmid, pGADCg
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159722 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGADCg
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Vector typeYeast Expression ; Yeast two-hybrid assay
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGID10
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SpeciesS. cerevisiae (budding yeast)
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Mutationremove stop codon
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Entrez GeneGID10 (a.k.a. YGR066C)
- Promoter ADH1
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Tags
/ Fusion Proteins
- 3xflag (C terminal on insert)
- Gal4AD (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCSJ392 was a gift from Alexander Varshavsky (Addgene plasmid # 159722 ; http://n2t.net/addgene:159722 ; RRID:Addgene_159722) -
For your References section:
Gid10 as an alternative N-recognin of the Pro/N-degron pathway. Melnykov A, Chen SJ, Varshavsky A. Proc Natl Acad Sci U S A. 2019 Aug 6;116(32):15914-15923. doi: 10.1073/pnas.1908304116. Epub 2019 Jul 23. 10.1073/pnas.1908304116 PubMed 31337681
