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p406TDH3
(Plasmid #15977)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15977 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS406
  • Backbone manufacturer
    Sikorski & Hieter, Genetics 122:19-27 (1989)
  • Backbone size w/o insert (bp) 4283
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TDH3 promoter + polylinker + CYC1 terminator
  • Alt name
    GPD, GLD1, HSP35, HSP36, SSS2 promoter
  • Alt name
    Glyceraldehyde-3-phosphate dehydrogenase
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    982
  • Mutation
    TDH3 promoter is between SacI and XbaI restriction sites.
  • Entrez Gene
    TDH3 (a.k.a. YGR192C, GLD1, HSP35, HSP36, SSS2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer M13 Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Plasmid was made by Ben Timney (Rockefeller University). Insert was cut from Mumberg plasmid p413GPD, which was obtained from ATCC.
  • Terms and Licenses

Depositor Comments

Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p406TDH3 was a gift from Nicolas Buchler & Fred Cross (Addgene plasmid # 15977)