|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15978||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5465
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer Gal4-Nterm (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
S. cerevisiae-E. coli shuttle vector. Cloning vector for two-hybrid system to express a GAL4 DBD-HA hybrid protein under the control of the ADH1 promoter. The 1.2-kb SacI/SalI fragment of pAS1 containing the ADH promoter, the GAL4 DNA-binding domain, and the HA epitope was inserted into the SacI/SalI sites of pRS404. Multi cloning sites from pBluescript II, f1(+) from pBluescript II.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAS404 was a gift from Eishi Noguchi (Addgene plasmid # 15978 ; http://n2t.net/addgene:15978 ; RRID:Addgene_15978)
For your References section:Saccharomyces cerevisiae putative G protein, Gtr1p, which forms complexes with itself and a novel protein designated as Gtr2p, negatively regulates the Ran/Gsp1p G protein cycle through Gtr2p. Nakashima N, Noguchi E, Nishimoto T. Genetics. 1999 Jul . 152(3):853-67. PubMed 10388807