V32M,L84M CoV-2 ORF8
(Plasmid
#159934)
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PurposeProtein overexpression in E.coli
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 159934 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbone2B-T
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCoV-2 ORF8 18-121
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SpeciesCoV-2
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MutationV32M, L84M
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Entrez GeneORF8 (a.k.a. GU280_gp09)
- Promoter T7
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Tag
/ Fusion Protein
- 6x Histidine, TEV cleavable (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
V32M,L84M CoV-2 ORF8 was a gift from James Hurley (Addgene plasmid # 159934 ; http://n2t.net/addgene:159934 ; RRID:Addgene_159934) -
For your References section:
Structure of SARS-CoV-2 ORF8, a rapidly evolving coronavirus protein implicated in immune evasion. Flower TG, Buffalo CZ, Hooy RM, Allaire M, Ren X, Hurley JH. bioRxiv August 27, 2020 10.1101/2020.08.27.270637