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V32M,L84M CoV-2 ORF8
(Plasmid #159934)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 159934 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CoV-2 ORF8 18-121
  • Species
    CoV-2
  • Mutation
    V32M, L84M
  • Entrez Gene
    ORF8 (a.k.a. GU280_gp09)
  • Promoter T7
  • Tag / Fusion Protein
    • 6x Histidine, TEV cleavable (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    V32M,L84M CoV-2 ORF8 was a gift from James Hurley (Addgene plasmid # 159934 ; http://n2t.net/addgene:159934 ; RRID:Addgene_159934)
  • For your References section:

    Structure of SARS-CoV-2 ORF8, a rapidly evolving coronavirus protein implicated in immune evasion. Flower TG, Buffalo CZ, Hooy RM, Allaire M, Ren X, Hurley JH. bioRxiv August 27, 2020 10.1101/2020.08.27.270637