V32M,L84M CoV-2 ORF8
(Plasmid #159934)

Purpose

Protein overexpression in E.coli
Depositing Lab

James Hurley
Publication

Flower et al bioRxiv August 27, 2020 ( How to cite )
Sequence Information
- Sequences (2)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	159934	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
2B-T
(Search Vector Database)
Vector type
Bacterial Expression

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
Low Copy

Gene/Insert

Gene/Insert name
CoV-2 ORF8 18-121
Species
CoV-2
Mutation
V32M, L84M
Entrez Gene
ORF8 (a.k.a. GU280_gp09)
Promoter T7
Tag / Fusion Protein
- 6x Histidine, TEV cleavable (N terminal on backbone)

Cloning Information

Cloning method Ligation Independent Cloning
5′ sequencing primer Unknown

(Common Sequencing Primers)

Resource Information

Terms and Licenses
- UBMTA
Industry Terms
- Not Available to Industry

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

V32M,L84M CoV-2 ORF8 was a gift from James Hurley (Addgene plasmid # 159934 ; http://n2t.net/addgene:159934 ; RRID:Addgene_159934)
For your References section:

Structure of SARS-CoV-2 ORF8, a rapidly evolving coronavirus protein implicated in immune evasion. Flower TG, Buffalo CZ, Hooy RM, Allaire M, Ren X, Hurley JH. bioRxiv August 27, 2020 10.1101/2020.08.27.270637

V32M,L84M CoV-2 ORF8 (Plasmid #159934)