pJMP2371
(Plasmid
#160078)
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PurposeZ. mobilis CRISPRi, promoter E, empty guide, sfGFP
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 160078 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneR6K_
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)BW25141
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameempty sgRNA
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gRNA/shRNA sequenceempty sgRNA
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SpeciesOther
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJMP2371 was a gift from Jason Peters (Addgene plasmid # 160078 ; http://n2t.net/addgene:160078 ; RRID:Addgene_160078) -
For your References section:
A High-Efficacy CRISPR Interference System for Gene Function Discovery in Zymomonas mobilis. Banta AB, Enright AL, Siletti C, Peters JM. Appl Environ Microbiol. 2020 Nov 10;86(23). pii: AEM.01621-20. doi: 10.1128/AEM.01621-20. Print 2020 Nov 10. 10.1128/AEM.01621-20 PubMed 32978126
