pRWJB004
(Plasmid #160112)

• Purpose: (Empty Backbone) Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;
• Depositing Lab: Detlef Weigel
• Publication: Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10.1186/s13007-018-0330-7. eCollection 2018.

Backbone

• Vector backbone: pZ001
• Backbone manufacturer: Lampropoulos et al
• Backbone size (bp): 4114
• Modifications to backbone: Cassettes of pro::pcoCas9 and At2S3::mcherry are engineered into the backbone; and the cassette of ccdB-Chloramphenicol is replaced with proLacZ::LacZ expressing unit; BamHI and NotI are inserted to change the promoter for driving Cas9
• Vector type: Bacterial Expression, Plant Expression, CRISPR
• Promoter: UBQ10
• Selectable markers: red fluorescence

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • pRWJB004_for_addgene.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry