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pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)
(Plasmid #160140)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 160140 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    JDS246 (Plasmid #43861)
  • Vector type
    Mammalian Expression ; in vitro transcription; T7 promoter

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
  • Alt name
    RTW2861
  • Species
    Synthetic
  • Promoter CMV and T7
  • Tag / Fusion Protein
    • NLS(nucleoplasmin)-3xHA-P2A-EGFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer oBK6928-CCAAGTCTCCACCCCATTGACG
  • 3′ sequencing primer oBK219-GGGAGTGGCACCTTCCAGGGTC
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-T7-AsCas12a-P2A-EGFP (RTW2861) was a gift from Keith Joung & Benjamin Kleinstiver (Addgene plasmid # 160140 ; http://n2t.net/addgene:160140 ; RRID:Addgene_160140)
  • For your References section:

    Scalable characterization of the PAM requirements of CRISPR-Cas enzymes using HT-PAMDA. Walton RT, Hsu JY, Joung JK, Kleinstiver BP. Nat Protoc. 2021 Feb 5. pii: 10.1038/s41596-020-00465-2. doi: 10.1038/s41596-020-00465-2. 10.1038/s41596-020-00465-2 PubMed 33547443