|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16075||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameProtein synthesis reporter
Alt nameCaMKII alpha
SpeciesM. musculus (mouse)
Insert Size (bp)4400
Entrez GeneCamk2a (a.k.a. CaMKII, R74975, mKIAA0968)
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The coding sequence of green fluorescent protein is flanked by the 5' and 3' untranslated regions from CAMKII-alpha, conferring both dendritic mRNA localization and translational regulation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1 5'-myr-dEGFP-3' was a gift from Erin Schuman (Addgene plasmid # 16075 ; http://n2t.net/addgene:16075 ; RRID:Addgene_16075)
For your References section:Dynamic visualization of local protein synthesis in hippocampal neurons. Aakalu G, Smith WB, Nguyen N, Jiang C, Schuman EM. Neuron. 2001 May . 30(2):489-502. 10.1016/S0896-6273(01)00295-1 PubMed 11395009