Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pD-MSP1D1
(Plasmid #161607)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 161607 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET 28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    for protein expression use strain with internal source of T7 polymerase e.g. BL21(DE3)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    D-MSP1D1
  • Alt name
    membrane scaffold protein
  • Alt name
    APOA1
  • Alt name
    PDB: 1AV1
  • Species
    synthetic gene
  • Insert Size (bp)
    640
  • Mutation
    mutant of MSP1D1; all tryptophans were replaced by phenylalanines
  • Promoter T7
  • Tag / Fusion Protein
    • 7-His tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco 1 (not destroyed)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pD-MSP1D1 was a gift from Stephen Sligar (Addgene plasmid # 161607 ; http://n2t.net/addgene:161607 ; RRID:Addgene_161607)
  • For your References section:

    Dark, Ultra-Dark and Ultra-Bright Nanodiscs for membrane protein investigations. McLean MA, Denisov IG, Grinkova YV, Sligar SG. Anal Biochem. 2020 Oct 15;607:113860. doi: 10.1016/j.ab.2020.113860. Epub 2020 Aug 1. 10.1016/j.ab.2020.113860 PubMed 32750355