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(Plasmid #161607)


Item Catalog # Description Quantity Price (USD)
Plasmid 161607 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pET 28a
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    for protein expression use strain with internal source of T7 polymerase e.g. BL21(DE3)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    membrane scaffold protein
  • Alt name
  • Alt name
    PDB: 1AV1
  • Species
    synthetic gene
  • Insert Size (bp)
  • Mutation
    mutant of MSP1D1; all tryptophans were replaced by phenylalanines
  • Promoter T7
  • Tag / Fusion Protein
    • 7-His tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco 1 (not destroyed)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pD-MSP1D1 was a gift from Stephen Sligar (Addgene plasmid # 161607 ; ; RRID:Addgene_161607)
  • For your References section:

    Dark, Ultra-Dark and Ultra-Bright Nanodiscs for membrane protein investigations. McLean MA, Denisov IG, Grinkova YV, Sligar SG. Anal Biochem. 2020 Oct 15;607:113860. doi: 10.1016/j.ab.2020.113860. Epub 2020 Aug 1. 10.1016/j.ab.2020.113860 PubMed 32750355