Addgene - GFP Snail WT Plasmid Data

Plasmid 16225: GFP Snail WT

Gene/insert name:
Snail
Insert size:
800
Species:
H. sapiens (human)
Entrez Gene:
SNAI1 (SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1)
Fusion protein or tag:
EGFP
Terminal:
N terminal on backbone
Vector backbone:
pEGFP-C2
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
EGFP-C List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Mien-Chie Hung
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Dual regulation of Snail by GSK-3beta-mediated phosphorylation in control of epithelial-mesenchymal transition. Zhou et al (Nat Cell Biol. 2004 Oct . 6(10):931-40. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16225" in your Materials and Methods section.