HA-Akt CA (myr-HA-Akt)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16244||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
MutationConstitutively active due to myristoylation sequence (MGSSKSKPK).
Entrez GeneAkt1 (a.k.a. Akt, LTR-akt, PKB, PKB/Akt, PKBalpha, Rac)
/ Fusion Proteins
- Myr (N terminal on insert)
- HA (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-PA-R (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byDr. Philip Tsichlis
Terms and Licenses
Also see references:
Cancer Res. 2000 Dec 15;60(24):6841-5.
Clin Cancer Res. 2004 Jun 1;10(11):3815-24.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HA-Akt CA (myr-HA-Akt) was a gift from Mien-Chie Hung (Addgene plasmid # 16244)
For your References section:HER-2/neu blocks tumor necrosis factor-induced apoptosis via the Akt/NF-kappaB pathway. Zhou BP, Hu MC, Miller SA, Yu Z, Xia W, Lin SY, Hung MC. J Biol Chem. 2000 Mar 17. 275(11):8027-31. 10.1074/jbc.275.11.8027 PubMed 10713122
Map uploaded by the depositor.