Addgene - HER2 WT Plasmid Data

Plasmid 16257: HER2 WT

Gene/insert name:
HER2
Insert size:
4400
Species:
H. sapiens (human)
Entrez Gene:
ERBB2 (CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1)
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGHrev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Mien-Chie Hung
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Upregulation of CXCR4 is essential for HER2-mediated tumor metastasis. Li et al (Cancer Cell. 2004 Nov . 6(5):459-69. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16257" in your Materials and Methods section.