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pUNIV-SIG-5HTR4D100A
(Plasmid #16312)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 16312 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUNIV
  • Backbone size w/o insert (bp) 5257
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    h5HT4b Serotonin Receptor (Rs1 D100A RASSL)
  • Alt name
    Rs1
  • Alt name
    5HT4
  • Alt name
    HTR4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1272
  • Mutation
    Added FLAG tag; Aspartic Acid 100 changed to Alanine.
  • Entrez Gene
    HTR4 (a.k.a. 5-HT4, 5-HT4R)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV promoter
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Plasmid backbone is from Drs. Wes Kroeze and Bryan Roth (Univ. of North Carolina, Chapel Hill, NC)
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUNIV-SIG-5HTR4D100A was a gift from Bruce Conklin (Addgene plasmid # 16312)
  • For your References section:

    Osteoblast expression of an engineered Gs-coupled receptor dramatically increases bone mass. Hsiao EC, Boudignon BM, Chang WC, Bencsik M, Peng J, Nguyen TD, Manalac C, Halloran BP, Conklin BR, Nissenson RA. Proc Natl Acad Sci U S A. 2008 Jan 29. 105(4):1209-14. 10.1073/pnas.0707457105 PubMed 18212126