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tau/pET29b
(Plasmid #16316)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16316 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET29b
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5370
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tau
  • Alt name
    tau protein
  • Alt name
    Tau T40
  • Alt name
    tau transcript variant 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • GenBank ID
    NM_005910
  • Entrez Gene
    MAPT (a.k.a. DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

For bacterial expression of human tau T40.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tau/pET29b was a gift from Peter Klein (Addgene plasmid # 16316 ; http://n2t.net/addgene:16316 ; RRID:Addgene_16316)
  • For your References section:

    Activation of the Wnt signaling pathway: a molecular mechanism for lithium action. Hedgepeth CM, Conrad LJ, Zhang J, Huang HC, Lee VM, Klein PS. Dev Biol. 1997 May 1. 185(1):82-91. 10.1006/dbio.1997.8552 PubMed 9169052