xfz8N/pCS2+
(Plasmid #16322)

• Depositing Lab: Peter Klein
• Publication: Deardorff et al Development. 1998 Jul . 125(14):2687-700.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Vector type: Vertebrate expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: frizzled-8
• Alt name: xfz8N
• Alt name: Nxfz8
• Alt name: N8
• Species: X. laevis (frog)
• Insert Size (bp): 730
• Mutation: Extracellular ligand binding domain of frizzled-8. Secreted wnt inhibitor.
• Entrez Gene: fzd8.S (a.k.a. XELAEV_18032854mg, FZD8, Fz-8, frizzled8, fz8, fzd8-a, fzd8-b, xfz8)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This construct contains the amino terminus of xfz8, which includes the signal sequence, cysteine-rich domain (CRD) and the linker region. It terminates just before the first transmembrane region. This plasmid does not contain a myc tag and is potent at causing enlarged cement gland and shortened axes. This construct was generated by removing a ClaI-XhoI fragment from Nxfz8/CS2MT. For sense transcripts, cut with NotI.