p8141 MSCV-P C-FlagHA 18E7 D10K
(Plasmid
#163306)
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PurposeExpresses HPV18 E7 D10K
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163306 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneMSCV
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Vector typeRetroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHPV18 E7 D10K
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SpeciesHuman papillomavirus type 18
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Insert Size (bp)315
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MutationD10K
- Promoter MSCV LTR
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Tag
/ Fusion Protein
- FlagHA (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pLXSN (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p8141 MSCV-P C-FlagHA 18E7 D10K was a gift from Elizabeth White (Addgene plasmid # 163306 ; http://n2t.net/addgene:163306 ; RRID:Addgene_163306) -
For your References section:
A Conserved Amino Acid in the C Terminus of Human Papillomavirus E7 Mediates Binding to PTPN14 and Repression of Epithelial Differentiation. Hatterschide J, Brantly AC, Grace M, Munger K, White EA. J Virol. 2020 Aug 17;94(17). pii: JVI.01024-20. doi: 10.1128/JVI.01024-20. Print 2020 Aug 17. 10.1128/JVI.01024-20 PubMed 32581101
