HIV7-Bcl2-CD28-CD3z-T2A-EGFR
(Plasmid
#163465)
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PurposeLentiviral vector encoding a Bim-specific chimeric antigen receptor with a synthetic Bcl2 binding element
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163465 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneHIV7
- Backbone size w/o insert (bp) 6853
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBim-specific CAR
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Alt nameBcl2-long_spacer-CD28tm-CD28icd-CD3zicd-T2A-EGFRt
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)3006
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HIV7-Bcl2-CD28-CD3z-T2A-EGFR was a gift from David Baker (Addgene plasmid # 163465 ; http://n2t.net/addgene:163465 ; RRID:Addgene_163465) -
For your References section:
Designed protein logic to target cells with precise combinations of surface antigens. Lajoie MJ, Boyken SE, Salter AI, Bruffey J, Rajan A, Langan RA, Olshefsky A, Muhunthan V, Bick MJ, Gewe M, Quijano-Rubio A, Johnson J, Lenz G, Nguyen A, Pun S, Correnti CE, Riddell SR, Baker D. Science. 2020 Sep 25;369(6511):1637-1643. doi: 10.1126/science.aba6527. Epub 2020 Aug 20. 10.1126/science.aba6527 PubMed 32820060
