pUASTLOTattB_eGFP::Dpp
(Plasmid #163702)

• Purpose: GFP-tagged version of Dpp (Decapentaplegic) under control of UAS and LexO enhancers
• Depositing Lab: Markus Affolter
• Publication: Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. doi: 10.1038/nature15712. Epub 2015 Nov 9.

Backbone

• Vector backbone: pUASTLOTattB
• Backbone manufacturer: Kanca, O. et.al., Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development (2014)
• Backbone size w/o insert (bp): 8900
• Total vector size (bp): 4653
• Vector type: Insect Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: decapentaplegic
• Alt name: BMP, TGF-β, shv, TGF-beta, shortvein
• Species: D. melanogaster (fly)
• Insert Size (bp): 2520
• Tag / Fusion Protein:
  • Tagged with eGFP (C terminal on insert)

Resource Information

• Supplemental Documents:
  • eGFP-Dpp.gb
• A portion of this plasmid was derived from a plasmid made by: The eGFP::DPP instert is based on the Dpp-GFP plasmid obtained from S. Cohen. Teleman, A. A. & Cohen, S. M. Dpp gradient formation in the Drosophila wing imaginal disc. Cell 103, 971–980 (2000).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For pUASTLOTattB_eGFP::Dpp, GFP was replaced by eGFP in the Dpp-GFP plasmid (obtained from S. Cohen). Then, eGFP::Dpp was inserted in the multiple cloning site of pUASTLOTattB vector by standard cloning procedures.

pUASTLOTattB vector:
Kanca, O., Caussinus, E., Denes, A. S., Percival-Smith, A. & Affolter, M. Raeppli: a
whole-tissue labeling tool for live imaging of Drosophila development.
Development 141, 472–480 (2014).

Original Dpp-GFP plasmid:
Teleman, A. A. & Cohen, S. M. Dpp gradient formation in the Drosophila wing
imaginal disc. Cell 103, 971–980 (2000).