pGGG-AH-VRT-A2
(Plasmid
#163703)
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PurposeWheat transformation vector pGoldenGreenGate (pGGG) with OsActinP:: hygromycin (hpt) selection and the full native Triticum polonicum VRT-A2 gene (native prom::genomic seq::3'UTR)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163703 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGoldenGreenGate (pGGG)
- Backbone size w/o insert (bp) 2831
- Total vector size (bp) 14901
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameOs Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
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SpeciesSynthetic
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Insert Size (bp)2892
- Promoter Rice - Os Actin promoter
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site Golden Gate BpiI (destroyed during cloning)
- 3′ cloning site Golden Gate BpiI (destroyed during cloning)
- 5′ sequencing primer LB Forward 5’-GTGGTGTAAACAAATTGACGC-3’
- 3′ sequencing primer Actin P Forward 5’-CGTATCAAAGTACCGACAAAAACA-3’ (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameTriticum polonicum VRT-A2 genomic sequence (Native promoter:: genomic sequence::3'UTR) + Nos Terminator
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SpeciesTriticum polonicum
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Insert Size (bp)8916
- Promoter Triticum polonicum VRT-A2
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site Golden Gate BsaI (destroyed during cloning)
- 3′ cloning site Golden Gate BsaI (destroyed during cloning)
- 5′ sequencing primer Actin P Forward 5’-CGTATCAAAGTACCGACAAAAACA-3’
- 3′ sequencing primer RB-R1 Reverse 5’-CCCGCCAATATATCCTGTCA-3’ (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.11.09.375154v2 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGGG-AH-VRT-A2 was a gift from Cristobal Uauy (Addgene plasmid # 163703 ; http://n2t.net/addgene:163703 ; RRID:Addgene_163703) -
For your References section:
Ectopic expression of Triticum polonicum VRT-A2 underlies elongated glumes and grains in hexaploid wheat in a dosage-dependent manner. Adamski NM, Simmonds J, Brinton JF, Backhaus AE, Chen Y, Smedley M, Hayta S, Florio T, Crane P, Scott P, Pieri A, Hall O, Barclay JE, Clayton M, Doonan JH, Nibau C, Uauy C. Plant Cell. 2021 Aug 13;33(7):2296-2319. doi: 10.1093/plcell/koab119. 10.1093/plcell/koab119 PubMed 34009390
