FH1-PNGT#1-mCherry
(Plasmid #164098)

• Purpose: Fluorescent reporter for genetic tracing of proneural Glioblastoma cell-state.
• Depositing Lab: Gaetano Gargiulo
• Publication: Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. doi: 10.1158/2159-8290.CD-20-0219. Epub 2020 Dec 23.

Backbone

• Vector backbone: FUGW
• Backbone manufacturer: David Baltimore (Addgene plasmid # 14883)
• Backbone size w/o insert (bp): 7956
• Vector type: Lentiviral, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PNGT#1-mCherry
• Species: H. sapiens (human)
• Insert Size (bp): 1882

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (unknown if destroyed)
• 3′ cloning site: BsrGI (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

From TCGA datasets, we annotated the genes specific to proneural glioblastoma-subtype and identified corresponding cis-regulatory elements to generate synthetic reporters to trace subtype identity.