pVG48
(Plasmid #164706)

• Purpose: rtTA under the control of PGK1 promoter
• Depositing Lab: Sahand Jamal Rahi
• Publication: Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi: 10.1038/s41467-023-38959-8.

Backbone

• Vector backbone: EZL105
• Backbone manufacturer: Zhou et al, Nature, 2018 PMID: 29562237
• Modifications to backbone: The light-inducible transcription factor EL222 has been replaced with rTA.
• Vector type: Yeast Expression
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PGK1pr - rtTA - CYC1t
• Species: S. cerevisiae (budding yeast)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pVG48.gb
• A portion of this plasmid was derived from a plasmid made by: rtTA was cloned from pCH043 which was a gift from Attila Becskei

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

yEVenus is a codon-optimized yEGFP variant with F46L, S65G, V68L, S72A, M153T, V163A, S175G, and T203Y mutations. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.