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pHR_SFFv_Her2-BFP_RHL027
(Plasmid #164823)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 164823 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHR'SIN
  • Backbone size w/o insert (bp) 9033
  • Total vector size (bp) 11791
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Extracellular and transmembrane region of ERBB2
  • Alt name
    HER2_ECD+TM(23-675)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1959
  • Entrez Gene
    ERBB2 (a.k.a. CD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, NEU, NGL, TKR1, VSCN2, c-ERB-2, c-ERB2, p185(erbB2))
  • Promoter SFFV
  • Tag / Fusion Protein
    • tagBFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR_SFFv_Her2-BFP_RHL027 was a gift from Wendell Lim (Addgene plasmid # 164823 ; http://n2t.net/addgene:164823 ; RRID:Addgene_164823)
  • For your References section:

    T cell circuits that sense antigen density with an ultrasensitive threshold. Hernandez-Lopez RA, Yu W, Cabral KA, Creasey OA, Lopez Pazmino MDP, Tonai Y, De Guzman A, Makela A, Saksela K, Gartner ZJ, Lim WA. Science. 2021 Mar 12;371(6534):1166-1171. doi: 10.1126/science.abc1855. Epub 2021 Feb 25. 10.1126/science.abc1855 PubMed 33632893