|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16497||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5010
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Gene/Insert nameSmad binding element (mutant)
Alt nameSBE x 3 (mutant)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
Three copies of the mutant Smad binding site (cctGTTTATACtatGTTTATACa) are cloned into pGL3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MBE6-Luc was a gift from Bert Vogelstein (Addgene plasmid # 16497)
For your References section:Human Smad3 and Smad4 are sequence-specific transcription activators. Zawel L, Dai JL, Buckhaults P, Zhou S, Kinzler KW, Vogelstein B, Kern SE. Mol Cell. 1998 Mar . 1(4):611-7. 10.1016/S1097-2765(00)80061-1 PubMed 9660945
Map uploaded by the depositor.