Addgene - pCI-neo beta catenin delta45 Plasmid Data

Plasmid 16520: pCI-neo beta catenin delta45

Gene/insert name:
beta catenin
Insert size:
2350
Species:
H. sapiens (human)
Entrez Gene:
CTNNB1 (OK/SW-cl.35, CTNNB, MRD19, armadillo)
Mutation:
Delta 45 (HCT116 mutation)
Vector backbone:
pCI-neo
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Cloning site 5':
XhoI
Site destroyed during cloning:
Yes
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bert Vogelstein
Terms and Licenses:
MTA

Comments:

HCT116 has a 3-bp deletion that removes one amino acid (Ser45).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC. Morin et al (Science. 1997 Mar 21. 275(5307):1787-90. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16520" in your Materials and Methods section.