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Plasmid 16566: pBV-Luc mut MBS1
  • c-MYC binding sites

  • H. sapiens (human)

  • MYC (MRTL, MYCC, bHLHe39, c-Myc)

  • Luciferase

  • C terminal on backbone

  • Mutant MBS1

  • pBV-Luc
    (Search Vector Database)

  • Vogelstein Lab

  • Mammalian Expression, Luciferase

  • 4867

  • KpnI

  • No

  • EcoRI

  • No

  • RVprimer3 List of Sequencing Primers

  • LucNrev

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (1)
  • View map

  • Bert Vogelstein

  • UBMTA
    Luciferase Limited Use Label License

Comments: 

Different combinations of oligonucleotide pairs (A+B, A+D, C+B, C+D) were annealed and converted to double-stranded fragments through one PCR cycle. These promoter fragments were subcloned into pBV-luc. Further polymerase-derived mutants were identified while sequencing the reporter constructs.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Identification of CDK4 as a target of c-MYC. Hermeking et al (Proc Natl Acad Sci U S A. 2000 Feb 29. 97(5):2229-34. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16566" in your Materials and Methods section.