-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Plasmid | 16588 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepCEP4
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 10400
-
Vector typeMammalian Expression
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePUMA
-
Alt namep53 upregulated modulator of apoptosis
-
SpeciesH. sapiens (human)
-
Insert Size (bp)600
-
Entrez GeneBBC3 (a.k.a. JFY-1, JFY1, PUMA)
-
Tag
/ Fusion Protein
- 2x HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
-
Terms and Licenses
-
Industry Terms
- Not Available to Industry
-
Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHA-PUMA was a gift from Bert Vogelstein (Addgene plasmid # 16588 ; http://n2t.net/addgene:16588 ; RRID:Addgene_16588) -
For your References section:
PUMA induces the rapid apoptosis of colorectal cancer cells. Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. Mol Cell. 2001 Mar . 7(3):673-82. 10.1016/S1097-2765(01)00213-1 PubMed 11463391