pHA-PUMA
(Plasmid #16588)

• Depositing Lab: Bert Vogelstein
• Publication: Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	16588	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pCEP4
  (Search Vector Database)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10400
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PUMA
• Alt name: p53 upregulated modulator of apoptosis
• Species: H. sapiens (human)
• Insert Size (bp): 600
• Entrez Gene: BBC3 (a.k.a. JFY-1, JFY1, PUMA)
• Tag / Fusion Protein:
  • 2x HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Terms and Licenses:
  • UBMTA
• Industry Terms:
  • Not Available to Industry
• Articles Citing this Plasmid:
  • 4 References