pAAV-CaMKII-mRuby2-4x128-mAGNET
(Plasmid #166021)

• Purpose: Expresses mRuby in CaMKII+ cells with low miRNA-128 expression
• Depositing Lab: Xue Han
• Publication: Keaveney et al J Neurosci. 2020 Dec 9;40(50):9576-9588. doi: 10.1523/JNEUROSCI.2570-19.2020. Epub 2020 Nov 6.

Backbone

• Vector backbone: AAV
• Total vector size (bp): 6239
• Vector type: Mammalian Expression, Mouse Targeting, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mRuby2 with 4 miR-128 binding sites
• Species: M. musculus (mouse)
• Insert Size (bp): 827
• Mutation: None
• GenBank ID:
• Tags / Fusion Proteins:
  • None
  • None

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that 3' ITR is not annotated on Addgene's plasmid map, however the plasmid can still be packaged and functions as expected.