Addgene - c-myc promoter (Frag A) Plasmid Data

Plasmid 16605: c-myc promoter (Frag A)

Gene/insert name:
c-myc promoter (Frag A)
Alt name:
MYC
Insert size:
1200
Species:
H. sapiens (human)
Entrez Gene:
MYC (MRTL, bHLHe39, c-Myc)
Fusion protein or tag:
luciferase
Terminal:
C terminal on backbone
Mutation:
FragA (XmnI->KpnI). The KpnI site is at -1194 on the promoter.
Vector backbone:
pBV-Luc
(Search Vector Database)
Backbone manufacturer:
Vogelstein Lab
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4867
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
LucNRev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bert Vogelstein
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

See Author's Map and article for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Identification of c-MYC as a target of the APC pathway. He et al (Science. 1998 Sep 4. 281(5382):1509-12. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16605" in your Materials and Methods section.