Addgene - IRES-puro-YFP Plasmid Data

Plasmid 16617: IRES-puro-YFP

Gene/insert name:
YFP
Alt name:
Yellow fluorescent protein
Insert size:
700
Vector backbone:
pIRESpuro2
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5200
Cloning site 5':
NheI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Bert Vogelstein
Terms and Licenses:
MTA

Comments:

This is an improved version of the vector used in the publication.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16617" in your Materials and Methods section.