pMeCP2miRFP670 pc3942
(Plasmid #167568)

• Purpose: The miRFP670-gene is ligated c-terminally to the rat meCP2-gene.
• Depositing Lab: Cristina Cardoso
• Publication: Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10.1080/19491034.2019.1688932.

Backbone

• Vector backbone: pmiRFP670-N1
• Backbone manufacturer: addgene # 79987
• Backbone size w/o insert (bp): 4960
• Total vector size (bp): 6472
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MeCp2
• Species: R. norvegicus (rat)
• Insert Size (bp): 1476
• Entrez Gene: Mecp2 (a.k.a. MECP2_e1)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: MfeI (not destroyed)
• 5′ sequencing primer: gtcagatccgctagc
• 3′ sequencing primer: caactagaatgcagtg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry