pKP372
(Plasmid #167593)

• Purpose: Express TonB frameshift of amphipathic helix
• Depositing Lab: Kathleen Postle
• Publication: Postle et al bioRxiv 2021.12.08.471779

Backbone

• Vector backbone: pACYC184
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tonB fs
• Species: Escherichia coli
• Mutation: tonB with frameshift of amphipathic helix

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The frame-shift of the TonB amphipathic helix was produced by deletion of bp 607 (G) and insertion of a new bp (T) following position 636 of the subsequent tonB open reading frame. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.