pKP1693
(Plasmid #167595)

• Purpose: Express FepA with His6 tag between residues 393 and 394 of the mature amino acid sequence . Please note that mutation numbers are not based on start codon of insert.
• Depositing Lab: Kathleen Postle
• Publication: Gresock et al J Bacteriol. 2017 Apr 25;199(10). pii: JB.00649-16. doi: 10.1128/JB.00649-16. Print 2017 May 15.

Backbone

• Vector backbone: pPro24
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FepA
• Species: Escherichia coli
• Mutation: His6 tag inserted after residue 393 in the mature FepA sequence of pKP1302. (His tag is between amino acids 415-416 based on start codon)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry